The EpiOcularTM Model
EpiOcular-Specific Features:
General MatTek Tissue Features:- Unsurpassed Long-Term Tissue Reproducibility -
Lot-to-Lot, Year-to-Year - 3-Dimensional, Highly Differentiated Tissues
- Metabolically, Mitotically Active Tissues
- Produced in Easily Handled Cell Culture Inserts
- Grown in Completely Serum-Free Media System
- Quantifiable, Objective Test Endpoints
- Cost Effective Alternative to Animal and Human Clinical Testing
NOTE: Link to EpiOcular Technical Specifications
MatTek's EpiOcular™ corneal model consists of normal, human-derived epidermal keratinocytes which have been cultured to form a stratified, squamous epithelium similar to that found in the cornea. The epidermal cells, which are cultured on specially prepared cell culture inserts using serum free medium, differentiate to form a multi-layered structure which closely parallels the corneal epithelium.
The EpiOcular tissue model exhibits in vivo-like morphological and growth characteristics which are uniform and highly reproducible. EpiOcular consists of highly organized basal cells which progressively flatten out as the apical surface of the tissue is approached, analogous to the normal in vivo corneal epithelium. EpiOcular is mitotically and metabolically active and releases many of the pro-inflammatory agents (cytokines) known to be important in ocular irritation and inflammation.
The protocols for using the EpiOcular System are clear and straightforward. EpiOcular has been utilized with several common tests of cytotoxicity and irritancy, including MTT, IL-1α, PGE2, LDH, and sodium fluorescein permeability. Technicians find EpiOcular's rigid substrate design easy to handle in routine repetitive testing environments and scientists find that they are able to perform discriminating tests due to the model's high level of reproducibility. In fact, "Sub-Draize" testing is possible with EpiOcular, allowing quantifiable discrimination among mild, milder, and mildest product formulations.
Various industrial and toxicology laboratories are actively seeking alternatives to whole animal testing. Cosmetic, household product, pharmaceutical and petrochemical companies have initiated in vitro toxicology testing to evaluate their raw materials and final product formulations. A growing body of data indicates that EpiOcular effectively provides a non-animal means to assess ocular irritation and other toxicological issues.
For all of these reasons, the EpiOcular model provides a predictive, morphologically relevant in vitro means to assess ocular irritancy.
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OCL-200 Prediction Equation
ET-50 (min) Figure 1: Determination of EpiOcular prediction
model -- Correlation of MTT ET-50 results to Draize rabbit eye data.
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ECETOC Materials (Figure 1)* |
ET-50
avg (min) |
Actual
Draize
(MMAS) |
1 |
Benzalkonium Chloride (1%) |
5.9 |
45.3 |
2 |
Benzalkonium Chloride (5%) |
1.0 |
83.8 |
3 |
Benzalkonium Chloride (10%) |
1.1 |
108.0 |
4 |
Cetyl Pyridinium Bromide (0.1%) |
>240 |
2.7 |
5 |
Cetyl Pyridinium Bromide (1%) |
30.1 |
36.0 |
6 |
Cetyl Pyridinium Bromide (10%) |
9.0 |
89.7 |
7 |
Glycerol |
>240 |
1.7 |
8 |
Propylene Glycol |
>240 |
1.3 |
9 |
Sodium Dodecyl Sulfate (3%) |
9.0 |
16.0 |
10 |
Sodium Dodecyl Sulfate (15%) |
5.1 |
59.2 |
11 |
Sodium Dodecyl Sulfate (30%) |
2.1 |
60.5 |
12 |
Sodium Hydroxide (1%) |
2.3 |
25.8 |
13 |
Sodium Hydroxide (10%) |
1.0 |
108.0 |
14 |
Tri-chloro acetic acid (3%) |
155.1 |
6.7 |
15 |
Tri-chloro acetic acid (30%) |
1.0 |
106.0 |
16 |
Triton X-100 (1%) |
36.7 |
1.7 |
17 |
Triton X-100 (5%) |
5.3 |
33.1 |
18 |
Triton X-100 (10%) |
2.5 |
68.7 |
19 |
Tween 20 (100%) |
>240 |
4.0 |
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Surfactants (Figure 1)* |
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20 |
Cetrimonium chloride |
116.9 |
6.7 |
21 |
Cocamide DEA |
>240 |
0.0 |
22 |
Disodium cocoamphodipropionate |
11.2 |
15.3 |
23 |
Na2-ricinoleadmido MEA sulfosuccinate |
108.9 |
0.0 |
24 |
Sodium cocoamphopropionate |
7.1 |
45.0 |
25 |
Sodium trideceth sulfate |
2.5 |
33.0 |
26 |
Stearalkonium chloride |
>240 |
14.0 |
27 |
Surfactant blend |
19.2 |
6.0 |
28 |
Surfactant blend |
40.4 |
2.7 |
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Consumer Products (Figure 1)* |
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29 |
10% fatty alcohol ethoxylate |
189.0 |
3.5 |
30 |
Body spray |
>240 |
0.0 |
31 |
Body/hand Wash |
9.1 |
32.0 |
32 |
Body/hand Wash |
14.8 |
35.0 |
33 |
Eye Gel/Colorant |
>240 |
0.0 |
34 |
Eye Gel/Colorant |
>240 |
2.0 |
35 |
Eye makeup remover (surfactant solution) |
>240 |
0.0 |
36 |
Face/body Wash |
>240 |
2.0 |
37 |
Face/body Wash |
6.5 |
25.0 |
38 |
Face/body Wash |
10.2 |
40.0 |
39 |
Final formulation shampoo |
63.1 |
3.5 |
40 |
Final formulation shampoo |
31.0 |
3.9 |
41 |
Final formulation shampoo |
26.1 |
4.0 |
42 |
Final formulation shampoo |
42.1 |
4.8 |
43 |
Final formulation shampoo |
29.4 |
6.6 |
44 |
Final formulation shampoo |
47.1 |
8.3 |
45 |
Final formulation shampoo |
29.1 |
12.5 |
46 |
Final formulation shampoo |
9.3 |
31.6 |
47 |
Final formulation shampoo |
4.2 |
32.7 |
48 |
Final formulation shampoo |
9.0 |
34.4 |
49 |
Hair conditioner |
>240 |
2.0 |
50 |
Hand/body Lotion |
>240 |
2.0 |
51 |
Hand/body Lotion |
>240 |
2.0 |
52 |
Hand/body Lotion |
>240 |
3.0 |
53 |
Hydroalcohol (air spray) solution |
84.1 |
6.0 |
54 |
Lactic acid (3%) |
>240 |
0.0 |
55 |
Oleic acid |
>240 |
2.0 |
56 |
Shampoo - baby |
30.8 |
10.0 |
57 |
Shampoo - baby |
25.7 |
18.0 |
58 |
Shampoo - regular |
6.0 |
30.0 |
59 |
Shampoo - regular |
8.7 |
35.0 |
60 |
Skin care emulsion |
>240 |
0.0 |
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Testing of OCL-200 Prediction
Model
In Vivo (MMAS) vs. Predicted Draize
In Vivo Draize (MMAS) Figure 2: Comparison of actual Draize to
predicted Draize score based on correlation equation (Figure 1). |
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Consumer Products (Figure 2)* |
Predicted
Draize |
Actual
Draize
(MMAS) |
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1 |
Body wash |
14.6 |
16.7 |
2 |
Body wash |
31.3 |
44.7 |
3 |
Dishwashing liquid |
51.3 |
38.3 |
4 |
Hand soap liquid |
28.0 |
24.7 |
5 |
Dishwashing liquid |
25.2 |
39.3 |
6 |
Facial soap |
15.6 |
9.3 |
7 |
Dishwashing liquid |
50.8 |
39.0 |
8 |
Dishwashing liquid |
60.5 |
50.3 |
9 |
Laundry detergent |
37.3 |
37.3 |
10 |
Laundry detergent |
1.8 |
0.7 |
11 |
Laundry detergent |
33.4 |
37.7 |
12 |
Dishwashing liquid |
96.9 |
37.7 |
13 |
Shampoo |
6.5 |
4.0 |
14 | Shampoo |
32.9 |
41.7 |
15 |
Shampoo |
8.4 |
3.3 |
16 |
Hand soap liquid |
18.1 |
13.3 |
17 |
Skin lotion |
1.8 |
0.7 |
18 |
Shampoo |
46.9 |
33.7 |
19 |
Skin lotion |
1.8 |
0.0 |
20 |
Shampoo |
39.3 |
37.7 |
21 |
Body wash |
41.2 |
33.0 |
22 |
Laundry detergent |
1.8 |
0.7 |
23 |
Laundry detergent |
39.9 |
44.0 |
24 |
Skin lotion |
1.8 |
0.7 |
* All materials diluted to 20% in ultrapure water prior to testing
(density > 0.95 g/ml).
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(Note: There are no In Vitro Product prices on this Web site.)
Please Also Review:
Guide to In Vitro Tissue Model Basics
EpiOcular Specification Sheet
EpiOcular Technical References
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