Dermal Phototoxicity
EpiDerm™ In Vitro Dermal Phototoxicity Measurement
The EpiDerm human skin tissue model was introduced over 15 years ago in response to demand for an in vitro (non-animal) alternative to the animal-based testing in the cosmetics, personal care, and household products industries.
EpiDerm Description
EpiDerm is a normal (non-transformed), human cell-derived, metabolically active, 3-dimensional organotypic in vitro skin model. Also known generically as reconstructed human epidermis (RhE), EpiDerm closely mimics human epidermis, both structurally and biochemically, and does so in a very reproducible manner. Proven EpiDerm dermal phototoxicity protocols, based on the well-documented MTT ET-50 Tissue Viability Assay, allow researchers to quantitatively measure the dermal phototoxicity of their experimental materials.
Types of materials that have been tested using the EpiDerm system include cosmetics and their constituents, household products, and pharmaceuticals (link to extensive list given below).
Major companies on the EpiDerm user list include Clairol, Johnson & Johnson, Procter & Gamble, Revlon, SmithKline Beecham and Unilever.
EpiDerm Dermal Phototoxicity Test Overview
Five concentrations of the test material (dissolved in H20 or oil or suspended in oil) are topically applied onto 2 EpiDerm Model EPI-200 tissues per concentration (i.e. 1 vehicle control + 5 concentrations = 12 tissues). A second set of 12 tissues is treated identically. Plates are incubated over night. Next day, one set of tissues is exposed to 6 J/cm² UVA (+UVA part of the test) and the other set is kept in the dark for the same period (-UVA part of the test). Tissues are then rinsed to remove test material, transferred to new 6 well plates with fresh medium and incubated overnight. Next day, assay medium is replaced by MTT-medium and tissues are incubated for 3 hours with MTT. Tissues are then rinsed and the formazan is extracted with isopropanol. Optical density is determined at 540/570 nm in a plate spectrophotometer and cell viability is calculated for each tissue as % of the corresponding vehicle control, either irradiated or unirradiated. A detailed test protocol is available--please contact MatTek to receive a copy.
PLEASE NOTE: MatTek has recently added the ability to introduce compounds to the tissue via the medium, thereby mimicking “systemic” dermal photosensitization caused by compounds either taken orally or by injection. A detailed test protocol is available--please contact MatTek to receive a copy.
EpiDerm Dermal Phototoxicity Technical References
Clicking on the link below will open a page that contains links to over 25 technical references describing the development, characterization, and use of this innovative human dermal phototoxicity measurement system. (Click on the TR number highlighted in blue to review that technical reference.)
Link to ALL EpiDerm Dermal Phototoxicity Technical References
Extensive List of Materials Tested
Click on the link below to view representative list of the materials MatTek and others have tested using the EpiDerm tissue model.
Materials Tested using EpiDerm
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EpiDerm Reconstructed Human Epidermis (RhE) Data Sheet
EpiDerm Product Specifications
EpiDerm Technical References
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