New MatTek Technical References October 2009

Listed below are the MatTek In Vitro Product Technical References added to the Web site in OCTOBER 2009. Click on the technical reference TITLE to review full citation information as well as a detailed summary.

• VISUALIZATION AND CHARACTERIZATION OF UVB-INDUCED REACTIVE OXYGEN SPECIES IN A HUMAN SKIN EQUIVALENT MODEL.
  
  SUMMARY:
  This study by researchers at Procter and Gamble, Kyoto University and Kyoto Pharmaceutical University demonstrated that MatTek’s EpiDerm in vitro 3-D human skin tissue equivalent is useful for visualizing the production of UVB-induced Reactive Oxygen Species (ROS) in real-time, and for evaluating the efficacy of topically applied anti-oxidative compounds to suppress ROS generation and attenuate sequential chemical and biological responses.
  
  
  
• Gamma-H2AX AS A BIOMARKER OF DNA DAMAGE INDUCED BY IONIZING RADIATION IN HUMAN PERIPHERAL BLOOD LYMPHOCYTES AND ARTIFICIAL SKIN.
  
  SUMMARY:
  This study by researchers at the National Cancer Institute (USA) demonstrated that MatTek’s EpiDermFT full-thickness in vitro 3-D human skin tissue equivalent is an excellent tissue model for performing experiments that measure the DNA damage caused by exposure to ionizing radiation because the tissue is derived from normal (non-transformed) human keratinocyes and fibroblasts.
  
  
  
• OXIDATIVE STRESS AND INFLAMMATORY RESPONSE IN DERMAL TOXICITY OF SINGLE-WALLED CARBON NANOTUBES.
  
  SUMMARY:
  This study by researchers at PPRB/NIOSH, West Virginia University and the University of Pittsburgh demonstrated that MatTek’s EpiDermFT full-thickness in vitro 3-D human skin tissue equivalent can be used to measure the dermal toxicity of single-walled carbon nanotubes (SWCNT).
  
  
  
• COLIPA VALIDATION OF THE RECONSTRUCTED HUMAN SKIN MICRONUCLEUS ASSAY (RSMN): A NOVEL MICRONUCLEUS ASSAY IN A 3D HUMAN SKIN MODEL.
  
  SUMMARY:
  This COLIPA-funded study by scientists at Procter and Gamble, Henkel AG and Co KGaA, L'Oreal, the Institute for In Vitro Sciences (IIVS), Unilever, Beiersdorf AG, KPSS (Kao Professional Salon Services), GlaxoSmithKline (GSK) and Pierre Fabre demonstrated that MatTek’s EpiDerm in vitro 3-D human skin tissue equivalent, when used as the basis for a human skin micronucleus assay (RSMN), provides a more realistic application/metabolism of test compounds for evaluating the genotoxicity of topically appliced cosmetics.
  
  
  
• DISRUPTION OF TIGHT JUNCTIONS BY CELLULOSE SULFATE FACILITATES HIV INFECTION: MODEL OF MICROBICIDE SAFETY.
  
  SUMMARY:
  This study by researchers in the Departments of Pediatrics, Microbiology-Immunology and Medicine at Albert Einstein College of Medicine demonstrated that MatTek’s EpiVaginal in vitro 3-D human cervico-vaginal tissue equivalent can be used to model potential epithelial barrier disruption caused by microbicide candidate formulations, and how epithelial barrier disruption leads to increased risk of HIV infection.
  
  
  
• RETAINING CELL INTEGRITY DURING ORGANOTYPIC MODEL VIABILITY ASSAYS: ALTERNATIVES TO MTT.
  
  SUMMARY:
  This study by researchers at the State University of New York (SUNY) at Stony Brook documents the effort to the develop an alternative tissue viability endpoint analysis using a non-lytic agent (MTS, Alamar Blue or CalceinAM) in place of the industry-standard MTT for MatTek’s EpiAirway in vitro 3-D human tracheal/bronchial tissue equivalent and MatTek’s EpiDerm in vitro 3-D human skin tissue equivalent, thereby allowing users to preserve the tissues on which the viability assay was performed, permitting subsequent assays to be performed on those same tissues.
  
  
  
• VALIDATING MTS AS AN ALTERNATIVE VIABILITY ASSAY TO MTT ON THE HUMAN 3-D TISSUE MODELS, EPIAIRWAY AND EPIDERM.
  
  SUMMARY:
  This study by researchers at the State University of New York (SUNY) at Stony Brook documents the development of an alternative tissue viability endpoint analysis using the non-lytic agent MTS in place of the industry-standard MTT for MatTek’s EpiAirway in vitro 3-D human tracheal/bronchial tissue equivalent and MatTek’s EpiDerm in vitro 3-D human skin tissue equivalent, thereby allowing users to preserve the tissues on which the viability assay was performed, permitting subsequent assays to be performed on those same tissues.
  
  
  
• REGULATION OF TIGHT JUNCTION PERMEABILITY BY SODIUM CAPRATE IN HUMAN KERATINOCYTES AND RECONSTRUCTED EPIDERMIS.
  
  SUMMARY:
  This study by researchers at Pola Chemical Industries Inc. and Jikei University School of Medicine used MatTek’s EpiDerm in vitro 3-D human skin tissue equivalent to investigate the effects of a known intestinal tissue tight junction dialator (sodium caprate) on human skin tissue tight junctions.
  
  
  
• REAWAKENING RETROCYCLINS: ANCESTRAL HUMAN DEFENSINS ACTIVE AGAINST HIV-1. (REPORT).
  
  SUMMARY:
  This study by researchers at the University of Massachusetts showed that MatTek’s EpiVaginal in vitro 3-D human cervico-vaginal tissue equivalent can be used to demonstrate the ability to restore endogenous expression of retrocyclin peptides.
  
  
  
• ANTIOXIDANT CAPACITY OF 3D HUMAN SKIN EPIDERM MODEL: EFFECTS ON SKIN MOISTURIZERS.
  
  SUMMARY:
  This study by researchers at North Dakota State University, the University of North Dakota and the Plastic Surgery Institute demonstrated how MatTek’s EpiDerm in vitro 3-D human skin tissue equivalent can be used to better understand the mechanisms of the effects of skin moisturizers containing antioxidant ingredients on skin function at the tissue level, and to establish effective strategies for skin protection and clinical treatments of skin disorders, and possibly healing wounds.
  
  
  
• EXPRESSION OF STRATUM CORNEUM LIPID PATHWAYS IN HUMAN SKIN EQUIVALENT CULTURES.
  
  SUMMARY:
  This study by scientists at Procter & Gamble Beauty demonstrated that gene pathways involved in stratum corneum lipid formation are expressed in MatTek’s EpiDermFT full-thickness in vitro 3-D human skin tissue equivalent, suggesting that ths culture system is an appropriate in vitro approach for studying stratum corneum biology.
  
  
  
• ANALYSIS OF IONIZING RADIATION-INDUCED DNA DAMAGE AND REPAIR IN THREE-DIMENSIONAL HUMAN SKIN MODEL SYSTEM.
  
  SUMMARY:
  This study by researchers in the Columbia University College of Physicians and Surgeons Center for Radiological Research demonstrated that MatTek’s EpiDerm in vitro 3-D human skin tissue equivalent and EpiDermFT full-thickness in vitro 3-D human skin tissue equivalent can be used to study the damaging effects to DNA of ionizing radiation exposure.
  
  
  
• THE TRANSPORT PROFILE OF CYTOKINES IN EPIDERMAL EQUIVALENTS SUBJECTED TO MECHANICAL LOADING.
  
  SUMMARY:
  This study by researchers at Eindhoven University of Technology and the University of London demonstrated how MatTek’s EpiDerm in vitro 3-D human skin tissue equivalent can be used to study the use of soft tissue cytokine release as potential biochemical markers for pressure ulcer risk assessment.
  
  
  
• POMEGRANATE DERIVED PRODUCTS FOR CANCER CHEMOPREVENTION.
  
  SUMMARY:
  This study by researchers in the University of Wisconsin's Dept. of Dermatology used MatTek’s EpiDermFT full-thickness in vitro 3-D human skin tissue equivalent because it "sustains differentiation and exhibits morphological and metabolic properties similar to human skin", to demonstrate the effect of a test substance applied to EpiDerm-FT to prevent skin cancer.
  
  
  
• USE OF HPLC/UPLC INSTEAD OF PHOTOMETRY FOR EVALUATION OF MTT IN IN VITRO IRRITATION ASSAYS BASED ON HUMAN RECONSTRUCTED TISSUE FOR ASSESSMENT OF COLOURED TEST MATERIALS.
  
  SUMMARY:
  This study by researchers at several Procter & Gamble research facilities in the European Union utilized MatTek’s EpiOcular in vitro 3-D human corneal tissue equivalent to demonstrate that the use of HPLC/UPLC technology can extend the applicability of the MTT reduction assay to test materials that are intrinsically coloured and that may interfere with evaluation of the MTT assay using standard photometry.
  
  
  
• EYE AND SKIN IRRITATION IN 3-D HUMAN TISSUE CONSTRUCTS USING MTT AND ATP ENDPOINTS.
  
  SUMMARY:
  This study by scientists at Beauty Avenues and the Institute for In Vitro Sciences (IIVS) demonstrated that MatTek’s EpiDerm in vitro 3-D human skin tissue equivalent and EpiOcular in vitro 3-D human corneal tissue equivalent can be used, if/when necessary, with the ATP tissue viability assay in addition to the industry-standard MTT tissue viability assay.
  
  
  
• MICROVESICATING EFFECTS OF SULFUR MUSTARD ON AN IN VITRO HUMAN SKIN MODEL.
  
  SUMMARY:
  This study by scientists at the US Army Medical Research Institute of Chemical Defense, University of Iowa, Harvard Medical School and MatTek Corp. demonstrated that MatTek's EpiDermFT full-thickness in vitro 3-D human skin tissue equivalent with a well-developed basement membrane structure is a useful tool for in vitro mechanistic studies of sulfur mustard vesicant (blistering) activity and development of preventive/therapeutic approaches for sulfur mustard pathology.