EpiDerm™ Reproducibility
The Best Again!"EpiDerm...the most reproducible model"
A very important EpiDerm-related study has just been published in Skin Pharmacology and Applied Skin Physiology (Vol. 15, Supplement 1, 2002). The Supplement, titled "Reconstructed Human Epidermis Equivalents, Characterization and Applications in Cutaneous Pharmacotoxicology", presents the results of a formal European Union study commissioned by EC DG XII, "TESTING AND IMPROVEMENT OF RECONSTRUCTED SKIN KITS IN ORDER TO ELABORATE EUROPEAN STANDARDS".
This comprehensive multi-year, multi-country study, headed by L'Oreal (France) and involving Beiersdorf (Germany), Wella Cosmital (Switzerland) and Leiden University (The Netherlands), characterized the skin models (tissue architecture and lipid composition) and assessed the models utility in skin irritation, percutaneous absorption and metabolism studies.
Performance Summary
Reproducibility of Cutaneous Irritancy Testing
| Skin Model |
ET-50 |
Avg. Intra-lot
CV* |
Avg. Inter-lot
CV* |
| EpiDerm™ |
3.02 |
10.1 |
20.4 |
| EpiSkin™ |
1.38 |
22.0 |
35.6 |
| Cosmital™ |
1.23 |
19.5 |
79.5 |
| Skinethic™ |
0.48 |
27.8 |
85.9 |
* Results based on Multi-Laboratory Validation study. This data was derived from Table 1, page 79 of Faller, C., et al., "Reconstructed skin kits: reproducibility of cutaneous irritancy testing", Skin Pharmacology and Applied Skin Physiology, 2002, 15, 74-91.
For the MTT assay, MatTek had the highest ET-50 (most resistant to damage by SDS) and the best intra-lot and inter-lot reproducibility (lowest coefficients of variation) while Skinethic had the lowest ET-50 and the highest intra-lot and inter-lot variability.
Click on the link below to view an abstract of the chapter detailing EpiDerm's superior reproducibility ("Reconstructed Skin Kits: Reproducibility of Cutaneous Irritancy Testing"). This finding is certainly no surprise to current users of the EpiDerm skin model, and will no doubt prove to be an important consideration for any researcher seeking highly reproducible results in cutaneous pharmacotoxicology studies.
Link to Article Abstract
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