556. ASSESSMENT OF DERMAL TOXICITY OF NANOSILICA USING CULTURED KERATINOCYTES, A HUMAN SKIN EQUIVALENT MODEL AND AN IN VIVO MODEL.

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Park¹, Y-H., Kim¹, J.N., Jeong¹, S.H., Choi¹, J.E., Lee², S-H., Choi¹, B.H., Lee³, J.P., Sohn³, K.H., Park³, K.L., Kim², M-K., Son¹, S.W. ¹Laboratory of Cell Signaling and Nanomedicine, Department of Dermatology and Division of Brain Korea 21 Project for Biomedical Science, Korea University College of Medicine, Seoul, South Korea. ²Department of Biochemistry & Molecular Biology, Korea University College of Medicine, Seoul, South Korea. ³National Institute of Toxicological Research, Seoul, South Korea. Toxicology, 267, 178–181, (2010).

Summary:
This study by researchers at Korea University College of Medicine and the National Institute of Toxicological Research (Korea) demonstrated how MatTek’s EpiDerm in vitro 3-D human skin tissue equivalent can be used to test the skin irritation potential of nanosilica and "provided more useful screening data than the conventional cell culture model on the relative toxicities of nanoparticles."

Assessments of skin irritation potentials are important aspects of the development of nanotechnology.

Nanosilica is currently being widely used for commercial purposes, but little literature is available on its skin toxicity and irritation potential. This study was designed to determine whether nanosilica has the potential to cause acute cutaneous toxicity, using cultured HaCaT keratinocytes (CHK), a human skin equivalent model (HSEM), and in vivo model.

Nanosilica was characterized by scanning electron microscopy. Researchers evaluated the cytotoxic effects of nanosilica on CHKs and the HSEM. In addition, they also investigated whether two commercially available nanosilicas with different sizes (7 and 10–20 nm) have different effects. To confirm in vitro results, researchers evaluated the irritation potentials of nanosilicas on rabbit skin.

Nanosilicas reduced the cell viabilities of CHKs in a dose-dependent manner. However, the HSEM revealed no irritation at 500 ug/ml of nanosilica. Furthermore, this result concurred with Draize skin irritation test findings.

The present study data indicate that nanosilica does not cause acute cutaneous irritation. Furthermore, this study shows that the HSEM used provides more useful screening data than the conventional cell culture model on the relative toxicities of nanoparticles.

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EpiDerm Reconstructed Human Epidermis (RhE) Data Sheet

EpiDerm Specifications

EpiDerm Technical References

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Applications: Irritation - skin, Nanotechnology, Nanotoxicology

Keywords: Draize skin irritation test, EPI-200, EpiDerm, HaCaT keratinocytes (CHK), MTT, Nanoparticles, Nanosilica, Nanotechnology, Skin irritation

Materials Tested: 1.0% Triton X-100, Nanoparticles, Nanosilica

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