398. GENETICALLY-ENGINEERED IL-10-SECRETING KERATINOCYTES CONDITION LANGERHANS CELLS TOWARDS A TOLERAGENIC PHENOTYPE.

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Prakasam, S., Jotwani, R., Taichman, L., Cutler, C.W. State University of New York - SUNY - Stony Brook, USA. Presented at the International Association for Dental Research, Brisbane Australia, June 28-July 1, (2006)

Summary:
This study by researchers at the State University of New York at Stony Brook demonstrated that MatTek’s Human Dendritic (Langerhans) Cells can be used to determine the influence of epithelial cells and cytokines on Langerhans cell activation and phenotype in response to microbial challenge.

Epidermal Langerhans cells (MatTek Corp.), “sentinels” of the epithelium, undergo increased mobilization from oral mucosa in lichen planus, hairy-leukoplakia and periodontitis, presumably towards lymph nodes. The ability of Langerhans cells to induce immunoregulatory (toleragenic) / immunostimulatory effector responses may depend on the keratinocytes/ epithelial cell microenvironment in which they are “educated”.

The objective of this study by researchers at the State University of New York at Stony Brook (USA) was to determine the influence of epithelial cells and cytokines on Langerhans cell activation / phenotype in response to microbial challenge.

Researchers hypothesized that Langerhans cells conditioned with IL-10-secreting epithelial cells will optimally blunt Langerhans cell activation in response to E.coli LPS (EcLPS).

Results suggested that the epithelial cells and IL-10 may play roles in conditioning Langerhans cells towards a tolerogenic phenotype by blunting of antigen presentation.

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Dendritic Cells Data Sheet

Dendritic Cells Specifications

Dendritic Cells Technical References

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Applications: Cytokine expression/analysis, Immunological research, Infectious disease research

Keywords: CD1á, CD80, CD83, CD86, Cytokines, Dendritic cells, E.coli, Epithelial cell (EC), IL-10, Immunoregulatory, Immunostimulatory, Langerhan cells (LCs), P. gingivalis, Sentinels, T cells, Toleragenic, Toleragenic phenotype

Endpoints: CD1á down regulation, CD86 down regulation

Materials Tested: Lipopolysaccharide (LPS), Retroviral vectors containing IL-10 gene

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