314. DOPAMINE TRANSPORT ACROSS RESPIRATORY EPITHELIUM.

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Chemuturi¹, N., Donovan¹, M., Klausner², M., and Hayden², P. ¹College of Pharmacy, University of Iowa, Iowa City, IA. ²MatTek Corporation, Ashland, MA. Poster presented at the American Association of Pharmaceutical Scientists (AAPS), Salt Lake City, UT, October 26-30, (2003).

Summary: Dopamine is a catecholamine neurotransmitter implicated in CNS related disorders such as Parkinson’s disease, schizophrenia and Huntington’s disease. It is known to be transported by the dopamine transporter (DAT) in the CNS and an organic cation transporter (OCT) peripherally. It is orally ineffective due to high first pass metabolism and its inability to cross the blood brain barrier. Intranasal dopamine administration has shown preferential uptake into the brain as compared to intravenous administration. Bovine respiratory explants and a novel human derived tracheo/bronchial tissue system were used to investigate the processes underlying dopamine transport.

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EpiAirway Data Sheet

EpiAirway Specifications

EpiAirway Technical References

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Applications: Drug delivery - intranasal, Metabolism - airway

Keywords: Amantadine HCl, Bovine respiratory explants, CNS, Catecholamine, DAT , Diffusion chamber, Dopamine (DOPA), Dopamine HCl, Dopamine transporter (DAT), EpiAirway, Flux studies, GBR 12935, Human derived tracheo/bronchial tissue system, Human traceheobronchial cell cultures, Immunohistochemistry, Intranasal, Metabolism, Mucosa, Nasal, Nasal drug delivery, Nasal glands, Neurotransmitter, Nomifensine maleate, OCT, OCT-2, Organic cation transporter (OCT), Permeability, Respiratory, Respiratory mucosa, TEER, Tracheo-bronchial

Endpoints: Flux Studies, HPLC, Immunohistochemistry, Permeability

Materials Tested: Amantadine HC1, Dopamine HC1, GBR 12935, Nomifensive maleate

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