276. THE USE OF STANDARDIZED HUMAN SKIN MODELS FOR CUTANEOUS PHARMACOTOXICOLOGY STUDIES.

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Roguet, R., L'Oreal, Life Sciences, Advanced Research Laboratories, Clichy, France. Skin Pharmacology and Applied Skin Physiology, 15,(Suppl 1),1-3,(2002).

Summary: Reconstructed skin and epidermis models are finding increasingly numerous applications in cosmetology and dermatology. In particular, they are currently employed to assess the tolerability and efficacy of raw materials and formulations, in conditions approaching those of normal use. Importantly, the use of such models greatly reduces the need for animal testing. Various models of reconstructed skin and epidermis have been developed [1-4] and some are now produced industrially [5-8]. They are used for the prediction of cutaneous irritancy and, to a lesser extent, percutaneous absorption and cutaneous metabolism. However, before being officially recognized as valid alternative methods and entering routine use, standard protocols must be developed to assess their reproducibility and performance as compared with in situ human studies.

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EpiDerm Data Sheet

EpiDerm Specifications

EpiDerm Technical References

Percutaneous Absorption (EpiDerm Application)

Skin Irritation (EpiDerm Application)

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Applications: Irritation - skin, Metabolism - skin, Percutaneous absorption/penetration, Pharmacotoxicology, Reproducibility - skin tissue models, Transdermal permeation/penetration

Keywords: Benzoic acid, Caffeine, Cutaneous absorption, Cutaneous irritancy, Cutaneous irritation, Cutaneous metabolism, Cutaneous toxicity, Cytochrome P450, Dermal irritancy, Dermal irritation, EpiDerm, EpiDermal differentiation, EpiSkin, Epidermis models, GOT, Glutathione S-transferase, Human skin models, IL-1a, In vitro testing, LDH, MTT, MTT ET-50 tissue viability assay, MTT assay, Mannitol, Metabolism, NAD(P)H, P 450, Percutaneous absorption, Permeation, Pharmacotoxicology, Reconstructed epidermis, Reconstructed human epidermis, Reconstructed skin, Reproducibility, Skin irritancy, Skin irritation

Endpoints: GOT, IL-1a, Interleukin-1a, LDH, MTT, MTT ET-50, MTT-50

Materials Tested: Benzoic acid, Caffeine, Mannitol

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