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TOPICAL N-ACETYL GLUCOSAMINE AND NIACINAMIDE INCREASE HYALURONAN IN VITRO.
Osborne, R., Mullins, L.A., and Robinson, L.R.
The Procter & Gamble Company, Cincinnati, Ohio USA.
Summary:
This study by researchers at Procter & Gamble Co. demonstrated how MatTek's EpiDermFT full-thickness in vitro human skin tissue equivalent can be used to study the expression of a major extracellular matrix component of human skin (hyaluronan) when N-acetyl glucosamine and niacinamide are applied to the apical surface of the tissues simulating topical application on human skin in vivo.
INTRODUCTION
Hyaluronan is a mucopolysaccharide in the epidermis responsible for maintaining overall hydration and elasticity of the skin. A decline in epidermal hyaluronan content occurs during aging, contributing to decreased turgidity and increased wrinkle formation. ISI-Acetyl-glucosamine is a precursor of hyaluronan, and niacinamide is an essential co-factor of hyaluronan synthesis. In the current work, engineered human skin equivalent cultures (EpiDermFT EFT-400, MatTek Corp., Ashland, MA) were used to determine effects of topical N-acetyl glucosamine and niacinamide on hyaluronan and collagen expression, and for prediction of improved appearance of aging facial skin.
CONCLUSIONS
- In in vitro human skin cultures, topical application of N-acetyl glucosamine and niacinamide stimulated hyaluronan synthesis.
- These treatments also led to an increase in collagen (procollagen-1) expression.
- Twice daily use of a facial moisturizer containing a combination of 2% N-acetyl glucosamine and 4% niacinamide for 4 to 8 weeks by women with moderate to severe fine lines and wrinkles reduced the appearance of facial fine lines and wrinkles, particularly in the eye area of the face.
- The in vitro hyaluronan results suggest that this effacement of fine lines and wrinkles is due at least in part to improved hydration of the skin.
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EpiDermFT Data Sheet
EpiDermFT Specifications
EpiDermFT Technical References
Applications: Anti-aging, Anti-wrinkle
Keywords: EFT-400, EpiDermFT, Fine line and wrinkle improvements, Hyaluronan, Procollagen 1, Skin elasticity, Skin hydration
Materials Tested: N-Acetyl-glucosamine, Niacinamide |
