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USE OF PROTEOMIC TECHNOLOGIES FOR DISCOVERY OF NEW MARKERS OF SKIN IRRITATION IN VITRO.
Fletcher1, S.T., and Baker2, V.A.
1Safety & Environmental Assurance Centre, Unilever, Bedfordshire, U.K. 2CuDoS Cellular Development Systems Ltd, BioCity Nottingham, U.K.
Presented at 2003 Society of Toxicology Meeting.
Keywords: Anion exchange, Calcium binding proteins, Cation exchange, Cutaneous irritancy, Cutaneous irritation, Cutaneous toxicity, Dermal irritancy, Dermal irritation, Desorption, EpiDerm, Epidermal proteins, Gene expression, Growth factors, Hsp 60, Hsp27, Human patch test, Human skin irritation, Interleukins, Ionization, Ionized proteins, Lysates, MTT, MTT ET-50 tissue viability assay, MTT assay, Mass spectrometry, Metallothioneins, Microarray, Molecular events, Occupational disease, Predictive methods, Protein profiling, Proteins/fingerprints, Proteomic, Skin irritancy, Skin irritation, Skin irritation response, Skin toxicity
Endpoints: 2 DGE, MTT assay, Mass spectrometry
Materials Tested: Benzalkonium chloride, Nonanoic acid, Phenol, Sodium lauryl sulfate
Summary: Analysis of epidermal proteins from irritant-treated in vitro epidermal models by proteomics is a promising approach to detect new markers of human skin irritation. These data provide a more comprehensive understanding of the mechanisms involved in the skin irritation response. It is possible that some of these may provide novel markers of skin irritation.
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EpiDerm Data Sheet
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Skin Irritation (EpiDerm Application)
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