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PERMEATION AND SKIN ABSORPTION: REPRODUCIBILITY OF VARIOUS INDUSTRIAL RECONSTRUCTED HUMAN SKIN MODELS.
Lotte C., Patouillet C., Zanini M., Messager A., Roguet R.,
Advanced Research Laboratories, L'Oreal Life Sciences, Aulnay-sous-Bois, France.
Skin Pharmacology and Applied Skin Physiology, 15(suppl 1),18-30, (2002).
Keywords: Caffeine, EpiDerm, EpiSkin, Lauric acid, Mannitol, Percutaneous absorption, Permeation, Skin absorption, SkinEthic
Summary: Human reconstructed skin models could be very useful tools to quantify percutaneous permeation and absorption. Before using such models, the reproducibility in the same batch and/or various batches, i.e. the relevance of the results obtained, must be verified. The reproducibility of 3 industrial models – EpiDerm™, Episkin® and SkinEthic® - was tested regarding the permeation and skin absorption of 3 topically applied compounds (with a large range of physicochemical properties): lauric acid, caffeine and mannitol. For all the models, the intra-batch reproducibility was greater than the interbatch reproducibility. According to the batches tested, the larger difference in terms of reproducibility between the 3 models was observed in the case of mannitol, a very poor permeant. In this case, the best reproducibility was observed with EpiDerm and Episkin. Moreover, the rank order of the 3 compounds applied, in terms of permeation and skin absorption, was the same as that expected from ex vivo human skin. Such results revealed human skin models as a promising means to test in vitro permeation and percutaneous absorption of topical products.
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