265.

PROTEOMIC AND MICROARRAY INVESTIGATION TO IDENTIFY POTENTIAL IN VITRO ENDPOINTS FOR SKIN IRRITATION. Fletcher¹, S.T., Fentem¹, J.H., Basketter¹, D.A., Kelsell², M.Philpott², D.P., and Baker¹, V.A. ¹SEAC Toxicology, Unilever R&D, Colworth, Bedfordshire, U.K. ²Centre for Cutaneous Research, QMW, University of London, U.K. Presented at the Annual Meeting of the Society of Investigative Dermatology, Los Angeles, CA, May 15-18, (2002).

Keywords: COLIPA, Cutaneous irritancy, Cutaneous irritation, Cutaneous toxicity, DNA, Dermal irritancy, Dermal irritation, EpiDerm, Epithelial, Gene expression, Genomic, HS1, Hsp27, Microarray, Peroxiredoxin 1, Proteomics, RNA, Serine protease inhibitor, Skin irritancy, Skin irritation

Summary: There is a need to investigate the mechanistic basis of the human skin irritation response if relevant in vitro test systems for the predictive identification of skin irritation are to be developed.
The aim of this work was to identify proteins (and genes encoding proteins) which may be regulated in response to skin irritation, following exposure of a reconstructed 3D model of human skin (EpiDerm™) to the skin irritant sodium lauryl sulphate (SLS).

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