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INITIAL RESULTS WITH EPI-201, A DEVELOPING IN VITRO MODEL OF HUMAN EPIDERMIS.
Klausner, M., Neal, P., Kubilus, J.
MatTek Corp., Ashland MA, USA.
J. Invest. Dermatol., 110(4), 558, Abstr. #513 (1998).
Keywords: Ascorbic acid, Barrier, properties formation, Differentiation, cellular, Epi-201/Epi-200 PD (partially differentiated), EpiDerm, Re-epithelialization, Surfactants, Surfactants, Triton X-100, Triton X-100, Wound healing, Wounds
Summary: EPI-201 is a stratified culture of proliferating and differentiating human keratinocytes which has the potential to develop into a state-of-the-art, fully kerati-nized model of human epidermis (EpiDerm™). This development is sensitive to conditions of culture and affords a means to study the effects of pharmacological agents, growth factors, temperature, pH, nutritional additives, and other culture parameters on epidermal differentiation. In the present study, when 10 ng/ml of epidermal growth factor (EGF) is supplied to EPI-201, the resulting tissue contains a granular layer which is thicker and more heavily populated with keratohyalin granules compared to standard EpiDerm. The deletion of EGF yields a thinner tissue with a decreased number granule-containing cells, although cornified cells are present. Retinoic acid (RA) at
1.5 X 10-8 M induces a structure with large intercellular spaces between the basal and upper layers and causes the swelling of spinous cells. These effects are decreased at lower RA levels and are absent at 5 X 10-10 M. Ascorbic acid (50 ug/ml), thought to improve ceramide synthesis and promote barrier development, produces tissue which has increased resistance to the action of a common surfactant, Triton X-100. These observations suggest that EPI-201 may be useful in studies of re-epithelialization, wound healing, and normal barrier development.
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