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MATTEK'S TISSUE MODELS: NEW DEVELOPMENTS.
Klausner, M.
MatTek Corp., Ashland, MA 01721.
Presented at International Business Conference, "Latest In Vitro Testing Methods for Skin Care, Cosmetics, and Household Products," Washington, D.C., (1997).
Keywords: Carcinogenesis, Cosmetic Personal Care Products, Cutaneous irritancy, Cutaneous irritation, Cutaneous toxicity, Dermal irritancy, Dermal irritancy testing, Dermal irritation, Differentiation, cellular, Draize prediction model, Draize scores, Draize tests, Endpoints, MTT, Epi-200, Epi-201/Epi-200 PD (partially differentiated), EpiDerm, EpiOcular, Eye irritation, MTT, MTT ET-50 tissue viability assay, MTT assay, Melanin, MelanoDerm, Melanocytes, Melanogenesis, Ocular irritancy testing, Ocular irritation, Ocular irritation testing, Oncogenes, c-fos, Pre-validation, Prediction model, Prevalidation, Skin irritancy, Skin irritation, Tumor suppressor genes, Tumor(s), Validation
Summary: MatTek commercially offers three organotypic tissue models for irritancy and mechanistic studies: 1) EpiDerm™, a highly differentiated, keratinocyte-only model of the epidermis, 2) MelanoDerm™, a second generation version of EpiDerm in which functioning melanocytes have been incorporated, and 3) EpiOcular™, a three-dimensional, non-cornified epithelial tissue model which has been useful in predicting ocular irritancy in vitro. Previously, correlation of EpiOcular results yielded a useful "prediction model" which consisted of an equation from which one could calculate a Draize score based on in vitro MTT EpiOcular results. However, certain classes of materials (e.g. alcohol containing) were shown to be over-predicted by the assay. In addition, other assay protocols have been proposed which did not involve dilution of the test materials. Results comparing three different assay protocols run head-to-head will be presented along with an alternative correlation for alcohol-containing materials. Regarding EpiDerm, new immuno-histochemical results show that the oncogene, c-fos, and the tumor suppressor gene, p-53, are expressed following treatments known to induce carcinogenesis in animal studies. In addition, a new “long life” maintenance medium for EpiDerm has been developed which maintains normal epidermal structure for up to 2 weeks following shipment. A recent addition to the EpiDerm line of tissues, EPI-201*, will be introduced. EPI-201 is a three-day-less-mature version of EpiDerm. Results with EPI-201 show that it can be cultured after storage and shipping to yield tissue which is histologically and functionally identical to standard EpiDerm. As such, it is believed that EPI-201 will prove to be a valuable tool to study modulators of epidermal differentiation.
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EpiDerm Data Sheet
EpiDerm Specifications
EpiDerm Technical References
Skin Irritation (EpiDerm Application)
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EpiOcular Data Sheet
EpiOcular Specifications
EpiOcular Technical References
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MelanoDerm Data Sheet
MelanoDerm Technical Specifications
MelanoDerm Technical References
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